Lab Exercise - Introduction to Radioimmunoassay
Center for Radiochemistry and Nuclear Materials
Department of Chemistry
To understand the technique of radioimmunoassay by performing a chemical ‘mock-up’ of the procedure.
Theoretical background for radioimmunoassay (read)
Questions for the Students
Radioactive iodine is potentially very toxic if ingested. Although amounts of activity in this experiment are very low, disposable gloves and eye protection should be worn to avoid direct contact. Under no circumstances should the 125I be used under oxidizing conditions when elemental iodine might be formed with a danger of inhalation.
1 mL autopipette
1 mL of 5 different antigen solutions
1 mL assay solution
14 gamma vials+lids
1 mL tips
10 mL buffer pH 6.9
1 mL antibody times 6
The preparation that the lab Supervisor needs to do to ready the lab.