Center for Radiochemistry and Nuclear Materials
Department of Chemistry
To understand the technique of radioimmunoassay by performing a chemical ‘mock-up’ of the procedure.
Explanation and Exercise Guide
Theoretical background for radioimmunoassay
Experimental procedure for Introduction to Radioimmunoassay
Questions for the Students
1. Which was the plot that gave the most sensitive answer?
2. What is the major source of error in this experiment?
3. What are the other minor sources of error?
Radioactive iodine is potentially very toxic if ingested. Although amounts of activity in this experiment are very low, disposable gloves and eye protection should be worn to avoid direct contact. Under no circumstances should the 125
I be used under oxidizing conditions when elemental iodine might be formed with a danger of inhalation.
1 mL autopipette
1 mL of 5 different antigen solutions
1 mL assay solution
14 gamma vials+lids
1 mL tips
10 mL buffer pH 6.9
1 mL antibody times 6
Preparation for the lab Supervisor
The preparation that the lab Supervisor needs to do to ready the lab.
Feedback from Users and Supervisors
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