Developed by

Center for Radiochemistry and Nuclear Materials
Department of Chemistry
Loughborough University

Learning Goals

To understand the technique of radioimmunoassay by performing a chemical ‘mock-up’ of the procedure.

Explanation and Exercise Guide


Theoretical background for radioimmunoassay

Experimental Procedure

Experimental procedure for Introduction to Radioimmunoassay

Questions for the Students



1. Which was the plot that gave the most sensitive answer?
2. What is the major source of error in this experiment?
3. What are the other minor sources of error?

Safety Aspects

Radioactive iodine is potentially very toxic if ingested. Although amounts of activity in this experiment are very low, disposable gloves and eye protection should be worn to avoid direct contact. Under no circumstances should the 125I be used under oxidizing conditions when elemental iodine might be formed with a danger of inhalation.


Waste Bucket
1 mL autopipette
Marker pen

1 mL of 5 different antigen solutions
1 mL assay solution
14 gamma vials+lids
1 mL tips
10 mL buffer pH 6.9
1 mL antibody times 6

Preparation for the lab Supervisor

The preparation that the lab Supervisor needs to do to ready the lab.

Feedback from Users and Supervisors

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