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Chemical yield of the analysis is determined by iron measurement of the 100 μL aliquot with AAS/ICP-MS/ICP-OES. Prepare iron standard (1, 2, 3, 5, 10 ppm) and measure them. Draw a standard curve. Measure your unknown sample and determine its iron content (ppm) from your standard curve. Calculate the yield (%). Take into account the dilution of the samples.